Orthogonal analysis reveals inconsistencies in cargo loading of extracellular vesicles.

ABSTRACT: Since extracellular vesicles (EVs) have emerged as a promising drug delivery system, diverse methods have been used to load them with active pharmaceutical ingredients (API) in preclinical and clinical studies. However, there is yet to be an engineered EV formulation approved for human use, a barrier driven in part by the intrinsic heterogeneity of EVs. API loading is rarely assessed in the context of single vesicle measurements of physicochemical properties but is likely administered in a heterogeneous fashion to the detriment of a consistent product. Here, we applied a suite of single-particle resolution methods to determine the loading of rhodamine 6G (R6G) surrogate cargo mimicking hydrophilic small molecule drugs across four common API loading methods: sonication, electroporation, fre

SUBMITTER: Lowe NM 

PROVIDER: S-EPMC11342351 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature