Her4.3⁺ radial glial cells maintain the brain vascular network through activation of Wnt signaling.

ABSTRACT: During vascular development, radial glial cells (RGCs) regulate vascular patterning in the trunk and contribute to the early differentiation of the blood-brain barrier. Ablation of RGCs results in excessive sprouting vessels or the absence of bilateral vertebral arteries. However, interactions of RGCs with later brain vascular networks after pattern formation remain unknown. Here, we generated a her4.3 transgenic line to label RGCs and applied the metronidazole/nitroreductase system to ablate her4.3⁺ RGCs. The ablation of her4.3⁺ RGCs led to the collapse of the cerebral vascular network, disruption of the blood-brain barrier, and downregulation of Wnt signaling. The inhibition of Wnt signaling resulted in the collapse of cerebral vasculature, similar to that caused by

SUBMITTER: Wang P 

PROVIDER: S-EPMC11342778 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature