PURE-seq identifies <i>Egr1</i> as a Potential Master Regulator in Murine Aging by Sequencing Long-Term Hematopoietic Stem Cells.

ABSTRACT: Single-cell transcriptomics is valuable for uncovering individual cell properties, particularly in highly heterogeneous systems. However, this technique often results in the analysis of many well-characterized cells, increasing costs and diluting rare cell populations. To address this, we developed PURE-seq (PIP-seq for Rare-cell Enrichment and Sequencing) for scalable sequencing of rare cells. PURE-seq allows direct cell loading from FACS into PIP-seq reactions, minimizing handling and reducing cell loss. PURE-seq reliably captures rare cells, with 60 minutes of sorting capturing tens of cells at a rarity of 1 in 1,000,000. Using PURE-seq, we investigated murine long-term hematopoietic stem cells and their transcriptomes in the context of hematopoietic aging, identifying Egr1 as a

SUBMITTER: Pan S 

PROVIDER: S-EPMC11343112 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature