Identification of In Vivo Internalizing Cardiac-Specific RNA Aptamers.

ABSTRACT:

Background

The pursuit of selective therapeutic delivery to target tissue types represents a key goal in the treatment of a range of adverse health issues, including diseases afflicting the heart. The development of new cardiac-specific ligands is a crucial step towards effectively targeting therapeutics to the heart.

Methods

Utilizing an ex vivo and in vivo SELEX approaches, we enriched a library of 2'-fluoro modified aptamers for ventricular cardiomyocyte specificity. Lead candidates were identified from this library, and their binding and internalization into cardiomyocytes was evaluated in both ex vivo and in vivo mouse studies.

Results

The ex vivo and in vivo SELEX processes generated an aptamer library with significant c

SUBMITTER: Narayan C 

PROVIDER: S-EPMC11343129 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature