Exploring the association between circulating endothelial protein C receptor and disease activity of rheumatoid arthritis in a pilot study.

ABSTRACT:

Objectives

To investigate whether circulating endothelial protein C receptor (EPCR) is associated with disease activity and inflammatory markers in rheumatoid arthritis.

Methods

Thirty-eight RA patients and 21 healthy controls (HC) were recruited via the A3BC biobank. Peripheral blood mononuclear cells and plasma were isolated from the blood of these participants. Plasma soluble (s)EPCR, IL-6, IL-17 and sCD14 were measured by enzyme-linked immunosorbent assay, cell membrane-associated (m)EPCR by flow cytometry; EPCR gene H3 single nucleotide polymorphism (SNP), which contributes to high plasma sEPCR levels, by PCR and DNA sequencing. Data were analysed using FlowJo10 and GraphPad Prism 10.

Results

RA patients had higher levels of mEPCR on T cells and plasma sEP

SUBMITTER: Xue M 

PROVIDER: S-EPMC11343369 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature