Codon switching of conserved Ser residues in coagulation and fibrinolytic proteases.

ABSTRACT:

Background

Unique among all amino acids, Ser is encoded by 2 sets of codons, TCN and AGY (N = any nucleotide, Y = pyrimidine), that cannot interconvert through single nucleotide substitutions. Both codons are documented at the essential residues S195 and S214 within the active site of serine proteases. However, it is not known how the codons interconverted during evolution because replacement of S195 or S214 by other amino acids typically results in loss of activity.

Objective

To characterize the prevalence of codon switching among essential and non-essential Ser residues in coagulation and fibrinolytic proteases from different vertebrate lineages.

Methods

TCN and AGY codon usage was analyzed in >550 sequences.

Results

Evolutionary pressure to preserve the codo

SUBMITTER: Stojanovski BM 

PROVIDER: S-EPMC11343676 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature