DNA methylation of IFI44L as a potential blood biomarker for childhood-onset systemic lupus erythematosus.

ABSTRACT:

Background

IFN-induced protein 44-like (IFI44L) promoter methylation has been demonstrated to serve as an effective blood diagnostic biomarker for adult-onset SLE. However, its utility as a diagnostic marker for childhood-onset SLE (cSLE) remains to be verified.

Methods

Initially, we conducted a differential analysis of gene methylation and mRNA expression patterns in cSLE whole blood samples obtained from the public GEO database to determine IFI44L gene expression and assess the methylation status at its CpG sites. Subsequently, we collected clinical whole blood samples from 49 cSLE patients and 12 healthy children, employing an HRM-qPCR-based IFI44L methylation detection technique to evaluate its diagnostic efficacy in pediatric clinical practice.

Results

A total of

SUBMITTER: Wang J 

PROVIDER: S-EPMC11343705 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature