Sex-dependent intra-islet structural rearrangements affecting alpha-to-beta cell interactions lead to adaptive enhancements of Ca²⁺ dynamics in prediabetic beta cells.

ABSTRACT:

Aims/hypothesis

Prediabetic pancreatic beta cells can adapt their function to maintain normoglycaemia for a limited period of time, after which diabetes mellitus will manifest upon beta cell exhaustion. Understanding sex-specific beta cell compensatory mechanisms and their failure in prediabetes (impaired glucose tolerance) is crucial for early disease diagnosis and individualised treatment. Our aims were as follows: (1) to determine the key time points of the progression from beta cells' functional adaptations to their failure in vivo; and (2) to mechanistically explain in vivo sex-specific beta cell compensatory mechanisms and their failure in prediabetes.

Methods

Islets from male and female transgenic Ins1^CreERT2-GCaMP3 mice were transplanted into the anterior

SUBMITTER: Visa M 

PROVIDER: S-EPMC11343800 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature