From resonance to chaos by modulating spatiotemporal patterns through a synthetic optogenetic oscillator.

ABSTRACT: Oscillations are a recurrent phenomenon in biological systems across scales, but deciphering their fundamental principles is very challenging. Here, we tackle this challenge by redesigning the wellcharacterised synthetic oscillator known as "repressilator" in Escherichia coli and controlling it using optogenetics, creating the "optoscillator". Bacterial colonies manifest oscillations as spatial ring patterns. When we apply periodic light pulses, the optoscillator behaves as a forced oscillator and we systematically investigate the properties of the rings under various light conditions. Combining experiments with mathematical modeling, we demonstrate that this simple oscillatory circuit can generate complex dynamics that are transformed into distinct spatial patterns. We report the observat

SUBMITTER: Park JH 

PROVIDER: S-EPMC11343849 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature