The extensive m⁵C epitranscriptome of Thermococcus kodakarensis is generated by a suite of RNA methyltransferases that support thermophily.

ABSTRACT: RNAs are often modified to invoke new activities. While many modifications are limited in frequency, restricted to non-coding RNAs, or present only in select organisms, 5-methylcytidine (m⁵C) is abundant across diverse RNAs and fitness-relevant across Domains of life, but the synthesis and impacts of m⁵C have yet to be fully investigated. Here, we map m⁵C in the model hyperthermophile, Thermococcus kodakarensis. We demonstrate that m⁵C is ~25x more abundant in T. kodakarensis than human cells, and the m⁵C epitranscriptome includes ~10% of unique transcripts. T. kodakarensis rRNAs harbor tenfold more m⁵C compared to Eukarya or Bacteria. We identify at least five RNA m⁵C methyltransferases (R5CMTs), and strains dele

SUBMITTER: Fluke KA 

PROVIDER: S-EPMC11344067 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature