Synonymous rpsH variants: the common denominator in Escherichia coli adapting to ionizing radiation.

ABSTRACT: Ionizing radiation (IR) in high doses is generally lethal to most organisms. Investigating mechanisms of radiation resistance is crucial for gaining insights into the underlying cellular responses and understanding the damaging effects of IR. In this study, we conducted a comprehensive analysis of sequencing data from an evolutionary experiment aimed at understanding the genetic adaptations to ionizing radiation in Escherichia coli. By including previously neglected synonymous mutations, we identified the rpsH c.294T > G variant, which emerged in all 17 examined isolates across four subpopulations. The identified variant is a synonymous mutation affecting the 30S ribosomal protein S8, and consistently exhibited high detection and low allele frequencies in all subpopulations.

SUBMITTER: Stemwedel K 

PROVIDER: S-EPMC11344242 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature