The impact of the carbohydrate-binding module on how a lytic polysaccharide monooxygenase modifies cellulose fibers.

ABSTRACT:

Background

In recent years, lytic polysaccharide monooxygenases (LPMOs) that oxidatively cleave cellulose have gained increasing attention in cellulose fiber modification. LPMOs are relatively small copper-dependent redox enzymes that occur as single domain proteins but may also contain an appended carbohydrate-binding module (CBM). Previous studies have indicated that the CBM "immobilizes" the LPMO on the substrate and thus leads to more localized oxidation of the fiber surface. Still, our understanding of how LPMOs and their CBMs modify cellulose fibers remains limited.

Results

Here, we studied the impact of the CBM on the fiber-modifying properties of NcAA9C, a two-domain family AA9 LPMO from Neurospora crassa, using both biochemical methods as well as newly developed mul

SUBMITTER: Stopamo FG 

PROVIDER: S-EPMC11344300 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature