Improved pokeweed genome assembly and early gene expression changes in response to jasmonic acid.

ABSTRACT:

Background

Jasmonic acid (JA) is a phytohormone involved in regulating responses to biotic and abiotic stress. Although the JA pathway is well characterized in model plants such as Arabidopsis thaliana, less is known about many non-model plants. Phytolacca americana (pokeweed) is native to eastern North Americana and is resilient to environmental stress. The goal of this study was to produce a publicly available pokeweed genome assembly and annotations and use this resource to determine how early response to JA changes gene expression, with particular focus on genes involved in defense.

Results

We assembled the pokeweed genome de novo from approximately 30 Gb of PacBio Hifi long reads and achieved an NG50 of ~ 13.2 Mb and a minimum 93.9% complete BUSCO score for gene annotat

SUBMITTER: Dougherty K 

PROVIDER: S-EPMC11344361 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature