The downregulation of tight junction proteins and pIgR in the colonic epithelium causes the susceptibility of EpCAM^+/- mice to colitis and gut microbiota dysbiosis.

ABSTRACT:

Background

The genetic factors play important roles on the pathogenesis of inflammatory bowel disease (IBD). EpCAM is highly expressed in the intestinal epithelium. It is still unclear if the decrease or somatic mutation of EpCAM could cause IBD.

Methods

The WT and EpCAM^+/- mice were administrated with DSS intermittently for nearly 8 weeks. The colon, liver and feces were harvested to check the morphological and histological changes, the expression of inflammatory genes and the gut microbiota via H&E staining, immunofluorescence, qPCR, western blot and 16S rDNA sequence assays.

Results

The DSS administration induced more serious inflammation in the colon of EpCAM^+/- mice than WT mice. Compared to DSS-induced WT mice, the transcriptional levels of

SUBMITTER: Nie Y 

PROVIDER: S-EPMC11345229 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature