Genome-wide mapping of the binding sites of myocyte enhancer factor 2A in chicken primary myoblasts.

ABSTRACT: Myocyte enhancer factor 2A (MEF2A) is a transcription factor that plays a critical role in cell proliferation, differentiation and apoptosis. In contrast to the wide characterization of its regulation mechanism in mammalian skeletal muscle, its role in chickens is limited. Especially, its wide target genes remain to be identified. Therefore, we utilized Cleavage Under Targets and Tagmentation (CUT&Tag) technology to reveal the genome-wide binding profile of MEF2A in chicken primary myoblasts thus gaining insights into its potential role in muscle development. Our results revealed that MEF2A binding sites were primarily distributed in intergenic and intronic regions. Within the promoter region, although only 8.87% of MEF2A binding sites were found, these binding sites were concentrated arou

SUBMITTER: Wang X 

PROVIDER: S-EPMC11345569 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature