Enhanced In Situ Spatial Proteomics by Effective Combination of MALDI Imaging and LC-MS/MS.

ABSTRACT: Highly specialized cells are fundamental for the proper functioning of complex organs. Variations in cell-type-specific gene expression and protein composition have been linked to a variety of diseases. Investigation of the distinctive molecular makeup of these cells within tissues is therefore critical in biomedical research. Although several technologies have emerged as valuable tools to address this cellular heterogeneity, most workflows lack sufficient in situ resolution and are associated with high costs and extremely long analysis times. Here, we present a combination of experimental and computational approaches that allows a more comprehensive investigation of molecular heterogeneity within tissues than by either shotgun LC-MS/MS or MALDI imaging alone. We applied our pipeline to th

SUBMITTER: Schafer F 

PROVIDER: S-EPMC11345593 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature