Deconvoluting Monomer- and Dimer-Specific Distance Distributions between Spin Labels in a Monomer/Dimer Mixture Using <i>T</i>₁-Edited DEER EPR Spectroscopy.

ABSTRACT: Double electron-electron resonance (DEER) EPR is a powerful tool in structural biology, providing distances between pairs of spin labels. When the sample consists of a mixture of oligomeric species (e.g., monomer and dimer), the question arises as to how to assign the peaks in the DEER-derived probability distance distribution to the individual species. Here, we propose incorporating an EPR longitudinal electron relaxation (T₁) inversion recovery experiment within a DEER pulse sequence to resolve this problem. The apparent T₁ between dipolar coupled electron spins measured from the inversion recovery time (τ_inv) dependence of the peak intensities in the T₁-edited DEER-derived probability P(r) distance distributi

SUBMITTER: Schmidt T 

PROVIDER: S-EPMC11345870 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature