Inflammatory Alterations to Renal Lymphatic Endothelial Cell Gene Expression in Mouse Models of Hypertension.

ABSTRACT:

Introduction

Hypertension (HTN) is a major cardiovascular disease that can cause and be worsened by renal damage and inflammation. We previously reported that renal lymphatic endothelial cells (LECs) increase in response to HTN and that augmenting lymphangiogenesis in the kidneys reduces blood pressure and renal pro-inflammatory immune cells in mice with various forms of HTN. Our aim was to evaluate the specific changes that renal LECs undergo in HTN.

Methods

We performed single-cell RNA sequencing. Using the angiotensin II-induced and salt-sensitive mouse models of HTN, we isolated renal CD31+ and podoplanin+ cells.

Results

Sequencing of these cells revealed three distinct cell types with unique expression profiles, including LECs. The number and transcriptional dive

SUBMITTER: McDermott JG 

PROVIDER: S-EPMC11345939 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature