Comparison of standard exponential and linear techniques to amplify small cDNA samples for microarrays.

ABSTRACT:

Background

The need to perform microarray experiments with small amounts of tissue has led to the development of several protocols for amplifying the target transcripts. The use of different amplification protocols could affect the comparability of microarray experiments.

Results

Here we compare expression data from Pinus taeda cDNA microarrays using transcripts amplified either exponentially by PCR or linearly by T7 transcription. The amplified transcripts vary significantly in estimated length, GC content and expression depending on amplification technique. Amplification by T7 RNA polymerase gives transcripts with a greater range of lengths, greater estimated mean length, and greater variation of expression levels, but lower average GC content, than those from PCR amplific

SUBMITTER: Wadenback J 

PROVIDER: S-EPMC1134654 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature