Identification, purification and characterization of a novel human blood protein with binding affinity for prostate secretory protein of 94 amino acids.

ABSTRACT: PSP94 (prostate secretory protein of 94 amino acids), an abundant protein within semen, has reported local functions within the reproductive tract and reported systemic functions. Mechanisms of action remain poorly understood, but binding to undefined molecules within the prostate, pituitary, testis and blood may initiate some of these actions. PSP94 serum measurements, especially of bound and free forms, have potential clinical utility in prostate cancer management. Identification of the binding molecules will help in the understanding of PSP94's action, and enable further development of PSP94 serum assays. PSPBP (PSP94-binding protein) was purified from human serum by ammonium sulphate fractionation, ion-exchange and affinity chromatography. The glycosylated protein ran as two bands on S

SUBMITTER: Reeves JR 

PROVIDER: S-EPMC1134678 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature