Efficient co-expression of a recombinant staphopain A and its inhibitor staphostatin A in Escherichia coli.

ABSTRACT: Staphopain A is a staphylococcal cysteine protease. Genes encoding staphopain A and its specific inhibitor, staphostatin A, are localized in an operon. Staphopain A is an important staphylococcal virulence factor. It is difficult to perform studies on its interaction with other proteins due to problems in obtaining a sufficient amount of the enzyme from natural sources. Therefore efforts were made to produce a recombinant staphopain A. Sequences encoding the mature form of staphopain A and staphostatin A were PCR-amplified from Staphylococcus aureus genomic DNA and cloned into different compatible expression vectors. Production of staphopain A was observed only when the enzyme was co-expressed together with its specific inhibitor, staphostatin A. Loss of the function mutations introduced w

SUBMITTER: Wladyka B 

PROVIDER: S-EPMC1134686 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature