Multiplexed in situ protein imaging using DNA-barcoded antibodies with extended hybridization chain reactions.

ABSTRACT: Antibodies have long served as vital tools in biological and clinical laboratories for the specific detection of proteins. Conventional methods employ fluorophore or horseradish peroxidase-conjugated antibodies to detect signals. More recently, DNA-conjugated antibodies have emerged as a promising technology, capitalizing on the programmability and amplification capabilities of DNA to enable highly multiplexed and ultrasensitive protein detection. However, the nonspecific binding of DNA-conjugated antibodies has impeded the widespread adoption of this approach. Here, we present a novel DNA-conjugated antibody staining protocol that addresses these challenges and demonstrates superior performance in suppressing nonspecific signals compared to previously published protocols. We further exten

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11347153 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature