The Peptidoglycan Recognition Protein 1 confers immune evasive properties on pancreatic cancer stem cells.

ABSTRACT:

Objective

Pancreatic ductal adenocarcinoma (PDAC) has limited therapeutic options, particularly with immune checkpoint inhibitors. Highly chemoresistant 'stem-like' cells, known as cancer stem cells (CSCs), are implicated in PDAC aggressiveness. Thus, comprehending how this subset of cells evades the immune system is crucial for advancing novel therapies.

Design

We used the KPC mouse model (LSL-Kras^G12D/+; LSL-Trp53^R172H/+; Pdx-1-Cre) and primary tumour cell lines to investigate putative CSC populations. Transcriptomic analyses were conducted to pinpoint new genes involved in immune evasion. Overexpressing and knockout cell lines were established with lentiviral vectors. Subsequent in vitro coculture assays, in vivo mouse and zebraf

SUBMITTER: Lopez-Gil JC 

PROVIDER: S-EPMC11347225 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature