Bioinformatics analysis of ERCC family in pan-cancer and ERCC2 in bladder cancer.

ABSTRACT:

Background

Single nucleotide polymorphisms (SNPs) in DNA repair genes can impair protein function and hinder DNA repair, leading to genetic instability and increased cancer risk. The Excision Repair Cross-Complementation (ERCC) family plays a crucial role in nucleotide excision repair, yet their comprehensive multi-omics characterization and roles in tumor prognosis and immune microenvironment remain unexplored.

Methods and materials

We performed bioinformatics analysis using publicly available data from 33 cancer types to investigate associations between ERCC gene expression, patient prognosis, and clinical features. We also validated the role of ERCC2 in bladder cancer through in vitro assays, including CCK-8, colony formation, wound healing, and Transwell assays.

SUBMITTER: Zhang S 

PROVIDER: S-EPMC11347307 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature