Random mutagenesis of bacterial luciferase: critical role of Glu175 in the control of luminescence decay.

ABSTRACT: Bacterial luciferases (LuxAB) can be readily classed as slow or fast decay luciferases based on their rates of luminescence decay in a single turnover assay. Luciferases from Vibrio harveyi and Xenorhabdus (Photorhabdus) luminescens have slow decay rates, and those from the Photobacterium genus, such as Photobacterium fisheri, P. phosphoreum and P. leiognathi, have rapid decay rates. By substitution of a 67-amino-acid stretch of P. phosphoreum LuxA in the central region of the LuxA subunit, the 'slow' X. luminescens luciferase was converted into a chimaeric luciferase with a significantly more rapid decay rate [Valkova, Szittner and Meighen (1999) Biochemistry 38, 13820-13828]. To understand better the role of specific residues in the classification of luciferases as slow and fast decay, w

SUBMITTER: Hosseinkhani S 

PROVIDER: S-EPMC1134731 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature