Single-cell analysis of chromatin and expression reveals age- and sex-associated alterations in the human heart.

ABSTRACT: Sex differences and age-related changes in the human heart at the tissue, cell, and molecular level have been well-documented and many may be relevant for cardiovascular disease. However, how molecular programs within individual cell types vary across individuals by age and sex remains poorly characterized. To better understand this variation, we performed single-nucleus combinatorial indexing (sci) ATAC- and RNA-Seq in human heart samples from nine donors. We identify hundreds of differentially expressed genes by age and sex and find epigenetic signatures of variation in ATAC-Seq data in this discovery cohort. We then scale up our single-cell RNA-Seq analysis by combining our data with five recently published single nucleus RNA-Seq datasets of healthy adult hearts. We find variation such

SUBMITTER: Read DF 

PROVIDER: S-EPMC11347658 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature