Rapid Examination of Nonprocessed Renal Cell Carcinoma Using Nonlinear Microscopy.

ABSTRACT:

Context.—

Histology, the traditional method of examining surgical tissue under a microscope, is a time-consuming process involving the fixation of tissue in formalin, dehydration, embedding in paraffin, and cutting into thin sections for hematoxylin-eosin (H&E) staining. Frozen section analysis is a faster alternative used in surgery to quickly evaluate tissue, but it has limitations, such as the size of the specimens that can be analyzed and difficulties with fatty and bony tissues.

Objective.—

To rapidly examine nonprocessed kidney tumors using nonlinear microscopy (NLM), a fluorescence microscopy technique that can rapidly visualize fresh or fixed, rapidly stained, nonprocessed tissue resembling H&E histology. This technology eliminates the need for fixation, embedding, m

SUBMITTER: Guzman-Arocho YD 

PROVIDER: S-EPMC11347715 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature