Extremely Sensitive Genetically Encoded Temperature Indicator Enabling Measurement at the Organelle Level.

ABSTRACT: Intracellular temperature is a fundamental parameter in biochemical reactions. Genetically encoded fluorescent temperature indicators (GETIs) have been developed to visualize intracellular thermogenesis; however, the temperature sensitivity or localization capability in specific organelles should have been further improved to clearly capture when and where intracellular temperature changes at the subcellular level occur. Here, we developed a new GETI, gMELT, composed of donor and acceptor subunits, in which cyan and yellow fluorescent proteins, respectively, as a Förster resonance energy transfer (FRET) pair were fused with temperature-sensitive domains. The donor and acceptor subunits associated and dissociated in response to temperature changes, altering the FRET efficiency. Consequently

SUBMITTER: Fukushima SI 

PROVIDER: S-EPMC11348412 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature