Enzyme-Free In-Situ Electrochemical Measurement Using a Porous Membrane Electrode for Glucose Transport into Cell Spheroids.

ABSTRACT: Microphysiological systems have attracted attention because of their use in drug screening. However, it is challenging to measure cell functions in real time using a device. In this study, we developed a cell culture device using a porous membrane electrode for in situ electrochemical glucose measurements for cell analysis. First, a porous membrane electrode was fabricated and electrochemically evaluated for enzyme-free glucose measurement. Subsequently, the glucose uptake of MCF-7 spheroids was evaluated using living spheroids, fixed spheroids, supernatants, and glucose transporter inhibitor-treated spheroids. Conventionally, the direct optical measurement of glucose uptake requires fluorescence-labeled glucose derivatives. In addition, the glucose uptake can be evaluated by measuring the

SUBMITTER: Utagawa Y 

PROVIDER: S-EPMC11348417 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature