Heterogenous Expression and Purification of Lipid II Flippase from Staphylococcus aureus.

ABSTRACT:

Background

Staphylococcus aureus is a common pathogen with strains that are resistant to existing antibiotics. MurJ from S. aureus (SaMurJ), an integral membrane protein functioning as Lipid II flippase, is a potential target for developing new antibacterial agents against this pathogen. Successful expression and purification of this protein shall be useful in the development of drugs against this target.

Objective

In this study, we demonstrated the optimized expression and purification procedures of SaMurJ, identified suitable detergent for extracting and solubilizing the protein, and examined the peptidisc system to generate a detergent-free environment.

Methods

SaMurJ fused with N-terminal ten-His tag was expressed without induction. Six detergents we

SUBMITTER: Zheng YY 

PROVIDER: S-EPMC11348468 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature