Secretome of brain microvascular endothelial cells promotes endothelial barrier tightness and protects against hypoxia-induced vascular leakage.

ABSTRACT: Cell-based therapeutic strategies have been proposed as an alternative for brain and blood vessels repair after stroke, but their clinical application is hampered by potential adverse effects. We therefore tested the hypothesis that secretome of these cells might be used instead to still focus on cell-based therapeutic strategies. We therefore characterized the composition and the effect of the secretome of brain microvascular endothelial cells (BMECs) on primary in vitro human models of angiogenesis and vascular barrier. Two different secretome batches produced in high scale (scHSP) were analysed by mass spectrometry. Human primary CD34⁺-derived endothelial cells (CD34⁺-ECs) were used as well as in vitro models of EC monolayer (CMECs) and blood-brain barrier (BBB). C

SUBMITTER: Loiola RA 

PROVIDER: S-EPMC11348522 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature