Vitamin B₁₂ as a source of variability in isotope effects for chloroform biotransformation by Dehalobacter.

ABSTRACT: Carbon and chlorine isotope effects for biotransformation of chloroform by different microbes show significant variability. Reductive dehalogenases (RDase) enzymes contain different cobamides, affecting substrate preferences, growth yields, and dechlorination rates and extent. We investigate the role of cobamide type on carbon and chlorine isotopic signals observed during reductive dechlorination of chloroform by the RDase CfrA. Microcosm experiments with two subcultures of a Dehalobacter-containing culture expressing CfrA-one with exogenous cobamide (Vitamin B₁₂, B12⁺) and one without (to drive native cobamide production)-resulted in a markedly smaller carbon isotope enrichment factor (ε_{C, bulk}) for B12^- (-22.1 ± 1.9‰) compared to B12⁺

SUBMITTER: Phillips E 

PROVIDER: S-EPMC11348799 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature