Connectomic reconstruction of a female Drosophila ventral nerve cord.

ABSTRACT: A deep understanding of how the brain controls behaviour requires mapping neural circuits down to the muscles that they control. Here, we apply automated tools to segment neurons and identify synapses in an electron microscopy dataset of an adult female Drosophila melanogaster ventral nerve cord (VNC)¹, which functions like the vertebrate spinal cord to sense and control the body. We find that the fly VNC contains roughly 45 million synapses and 14,600 neuronal cell bodies. To interpret the output of the connectome, we mapped the muscle targets of leg and wing motor neurons using genetic driver lines² and X-ray holographic nanotomography³. With this motor neuron atlas, we identified neural circuits that coordinate leg and wing movements during take-off. We

SUBMITTER: Azevedo A 

PROVIDER: S-EPMC11348827 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature