DNA methylation and gene expression profiling reveal potential association of retinol metabolism related genes with hepatocellular carcinoma development.

ABSTRACT:

Background

Aberrant DNA methylation patterns play a critical role in the development of hepatocellular carcinoma (HCC). However, the molecular mechanisms associated with these aberrantly methylated genes remain unclear. This study aimed to comprehensively investigate the methylation-driven gene expression alterations in HCC using a multi-omics dataset.

Methods

Whole genome bisulfite sequencing (WGBS) and RNA sequencing (RNA-seq) techniques were used to assess the methylation and gene expression profiles of HCC tissues (HCCs) and normal adjacent tissues (NATs). The candidate genes' potential function was further investigated using single-cell RNA sequencing (scRNA seq) data.

Results

We observed widespread hypomethylation in HCCs compared to NATs. Methylation levels in

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC11348899 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature