Long-term passage impacts human dental pulp stem cell activities and cell response to drug addition in vitro.

ABSTRACT:

Background

Dental pulp stem cells (DPSCs) possess mesenchymal stem cell characteristics and have potential for cell-based therapy. Cell expansion is essential to achieve sufficient cell numbers. However, continuous cell replication causes cell aging in vitro, which usually accompanies and potentially affect DPSC characteristics and activities. Continuous passaging could alter susceptibility to external factors such as drug treatment. Therefore, this study sought to investigate potential outcome of in vitro passaging on DPSC morphology and activities in the absence or presence of external factor.

Methods

Human DPSCs were subcultured until reaching early passages (P5), extended passages (P10), and late passages (P15). Cells were evaluated and compared for cell an

SUBMITTER: Patntirapong S 

PROVIDER: S-EPMC11348901 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature