Cell derived matrices from bovine corneal endothelial cells as a model to study cellular dysfunction.

ABSTRACT:

Purpose

Fuchs endothelial corneal dystrophy (FECD) is a progressive corneal disease that impacts the structure and stiffness of the Descemet's membrane (DM), the substratum for corneal endothelial cells (CECs). These structural alterations of the DM could contribute to the loss of the CECs resulting in corneal edema and blindness. Oxidative stress and transforming growth factor-β (TGF-β) pathways have been implicated in endothelial cell loss and endothelial to mesenchymal transition of CECs in FECD. Ascorbic acid (AA) is found at high concentrations in FECD and its impact on CEC survival has been investigated. However, how TGF-β and AA effect the composition and rigidity of the CEC's matrix remains unknown.

Methods

In this study, we investigated the effect of AA, TGF-β1 and

SUBMITTER: Jalilian I 

PROVIDER: S-EPMC11349083 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature