Investigating Communication Dynamics in Neuronal Network using 3D Gold Microelectrode Arrays.

ABSTRACT: Although in vitro neuronal network models hold great potential for advancing neuroscience research, with the capacity to provide fundamental insights into mechanisms underlying neuronal functions, the dynamics of cell communication within such networks remain poorly understood. Here, we develop a customizable, polymer modified three-dimensional gold microelectrode array with sufficient stability for high signal-to-noise, long-term, neuronal recording of cultured networks. By using directed spatial and temporal patterns of electrical stimulation of cells to explore synaptic-based communication, we monitored cell network dynamics over 3 weeks, quantifying communication capability using correlation heatmaps and mutual information networks. Analysis of synaptic delay and signal speed be

SUBMITTER: Zhang K 

PROVIDER: S-EPMC11349149 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature