Iron starvation results in up-regulation of a probable Haloferax volcanii siderophore transporter.

ABSTRACT: The response of the haloarchaeal model organism Haloferax volcanii to iron starvation was analyzed at the proteome level by data-independent acquisition mass spectrometry. Cells grown in minimal medium with normal iron levels were compared to those grown under low iron conditions, with samples being separated into membrane and cytoplasmic fractions in order to focus on import/export processes which are frequently associated with metal homeostasis. Iron starvation not only caused a severe retardation of growth but also altered the levels of many proteins. Using a comprehensive annotated spectral library and data-independent acquisition mass spectrometry (DIA-MS), we found that iron starvation resulted in significant changes to both the membrane and the soluble proteomes of Hfx. vo

SUBMITTER: Sailer AL 

PROVIDER: S-EPMC11349517 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature