Fast formation and maturation enhancement of human liver organoids using a liver-organoid-on-a-chip.

ABSTRACT: Background: Spatial and functional hepatic zonation, established by the heterogeneous tissue along the portal-central axis of the liver, is important for ensuring optimal liver function. Researchers have attempted to develop reliable hepatic models to mimic the liver microenvironment and analyze liver function using hepatocytes cultured in the developed systems. However, mimicking the liver microenvironment in vitro remains a great challenge owing to the lack of perfusable vascular networks in the model systems and the limitation in maintaining hepatocyte function over time. Methods: In this study, we established a microphysiological system that operated under continuous flush medium flow, thereby allowing the supply of nutrients and oxygen to liver organoids and the r

SUBMITTER: Byeon JH 

PROVIDER: S-EPMC11349704 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature