A complete chromosome substitution mapping panel reveals genome-wide epistasis in Arabidopsis.

ABSTRACT: Chromosome substitution lines (CSLs) are tentatively supreme resources to investigate non-allelic genetic interactions. However, the difficulty of generating such lines in most species largely yielded imperfect CSL panels, prohibiting a systematic dissection of epistasis. Here, we present the development and use of a unique and complete panel of CSLs in Arabidopsis thaliana, allowing the full factorial analysis of epistatic interactions. A first comparison of reciprocal single chromosome substitutions revealed a dependency of QTL detection on different genetic backgrounds. The subsequent analysis of the complete panel of CSLs enabled the mapping of the genetic interactors and identified multiple two- and three-way interactions for different traits. Some of the detected epistatic effects we

SUBMITTER: Wijnen CL 

PROVIDER: S-EPMC11350127 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature