A broad-spectrum multiepitope vaccine against seasonal influenza A and B viruses in mice.

ABSTRACT:

Background

Influenza viruses pose a persistent threat to global public health, necessitating the development of innovative and broadly effective vaccines.

Methods

This study focuses on a multiepitope vaccine (MEV) designed to provide broad-spectrum protection against different influenza viruses. The MEV, containing 19 B-cell linear epitopes, 7 CD4⁺ T cells, and 11 CD8⁺ T cells epitopes identified through enzyme-linked immunospot assay (ELISPOT) in influenza viruses infected mice, was administered through a regimen of two doses of DNA vaccine followed by one dose of a protein vaccine in C57BL/6 female mice.

Findings

Upon lethal challenge with both seasonal circulating strains (H1N1, H3N2, BV, and BY) and historical strains (H1N1-PR8 and H3N2-X31),

SUBMITTER: Yuan L 

PROVIDER: S-EPMC11350506 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature