Discovery and demonstration of the temperature stress response functions of Dermatophagoides farinae proteins 1 and 2.

ABSTRACT:

Background

Dermatophagoides farinae proteins (DFPs) are abundantly expressed in D. farinae; however, their functions remain unknown. Our previous transcriptome sequencing analyses revealed that the basal expression of DFP1 and DFP2 in D. farinae was high and, more importantly, upregulated under temperature stress. Therefore, DFPs were speculated to exert a temperature stress response function.

Results

Real-time quantitative polymerase chain reaction detection revealed that both DFP1 and DFP2 were significantly upregulated under temperature stress. Particularly, DFP1 was upregulated under cold stress. Electrophoresis of D. farinae total proteins revealed an increased abundance of DFP1 and DFP2 (40-55 kDa bands) under temperature stress, which was corroborated by the mass spec

SUBMITTER: Zhang W 

PROVIDER: S-EPMC11351518 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature