Pulsed Dipolar EPR for Self-Limited Complexes of Oligonucleotides Studies.

ABSTRACT: Pulsed electron-electron double resonance (PELDOR) spectroscopy is a powerful method for determining nucleic acid (NA) structure and conformational dynamics. PELDOR with molecular dynamics (MD) simulations opens up unique possibilities for defining the conformational ensembles of flexible, three-dimensional, self-assembled complexes of NA. Understanding the diversity and structure of these complexes is vital for uncovering matrix and regulative biological processes in the human body and artificially influencing them for therapeutic purposes. To explore the reliability of PELDOR and MD simulations, we site-specifically attached nitroxide spin labels to oligonucleotides, which form self-assembled complexes between NA chains and exhibit significant conformational flexibility. The DNA complexe

SUBMITTER: Chubarov AS 

PROVIDER: S-EPMC11351890 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature