A General Method to Screen Nanobodies for Cytochrome P450 Enzymes from a Yeast Surface Display Library.

ABSTRACT: The availability of yeast surface display nanobody (Nb) libraries offers a convenient way to acquire antigen-specific nanobodies that may be useful for protein structure-function studies and/or therapeutic applications, complementary to the conventional method of acquiring nanobodies through immunization in camelids. In this study, we developed a general approach to select nanobodies for cytochrome P450 enzymes from a highly diverse yeast display library. We tested our method on three P450 enzymes including CYP102A1, neuronal nitric oxide synthase (nNOS), and the complex of CYP2B4:POR, using a novel streamlined approach where biotinylated P450s were bound to fluorescent-labeled streptavidin for Nb screening. The Nb-antigen binders were selectively enriched using magnetic-activated cell sor

SUBMITTER: Sun Y 

PROVIDER: S-EPMC11351928 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature