Regulatory Effects of Senescent Mesenchymal Stem Cells: Endotheliocyte Reaction.

ABSTRACT: Currently, there is a growing focus on aging and age-related diseases. The processes of aging are based on cell senescence, which results in changes in intercellular communications and pathological alterations in tissues. In the present study, we investigate the influence of senescent mesenchymal stem cells (MSCs) on endothelial cells (ECs). In order to induce senescence in MSCs, we employed a method of stress-induced senescence utilizing mitomycin C (MmC). Subsequent experiments involved the interaction of ECs with MSCs in a coculture or the treatment of ECs with the secretome of senescent MSCs. After 48 h, we assessed the EC state. Our findings revealed that direct interaction led to a decrease in EC proliferation and migratory activity of the coculture. Furthermore, there was an increas

SUBMITTER: Ratushnyy A 

PROVIDER: S-EPMC11352319 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature