Actin Cytoskeleton and Integrin Components Are Interdependent for Slit Diaphragm Maintenance in <i>Drosophila</i> Nephrocytes.

ABSTRACT: In nephrotic syndrome, the podocyte filtration structures are damaged in a process called foot process effacement. This is mediated by the actin cytoskeleton; however, which actins are involved and how they interact with other filtration components, like the basement membrane, remains poorly understood. Here, we used the well-established Drosophila pericardial nephrocyte-the equivalent of podocytes in flies-knockdown models (RNAi) to study the interplay of the actin cytoskeleton (Act5C, Act57B, Act42A, and Act87E), alpha- and beta-integrin (basement membrane), and the slit diaphragm (Sns and Pyd). Knockdown of an actin gene led to variations of formation of actin stress fibers, the internalization of Sns, and a disrupted slit diaphragm cortical pattern. Notably, deficiency of Act

SUBMITTER: Delaney M 

PROVIDER: S-EPMC11352372 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature