Cl^--dependent amplification of excitatory synaptic potentials at distal dendrites revealed by voltage imaging.

ABSTRACT: The processing of synaptic signals in somatodendritic compartments determines neuronal computation. Although the amplification of excitatory signals by local voltage-dependent cation channels has been extensively studied, their spatiotemporal dynamics in elaborate dendritic branches remain obscure owing to technical limitations. Using fluorescent voltage imaging throughout dendritic arborizations in hippocampal pyramidal neurons, we demonstrate a unique chloride ion (Cl^-)-dependent remote computation mechanism in the distal branches. Excitatory postsynaptic potentials triggered by local laser photolysis of caged glutamate spread along dendrites, with gradual amplification toward the distal end while attenuation toward the soma. Tour de force subcellular patch-clamp recordings fr

SUBMITTER: Higashi R 

PROVIDER: S-EPMC11352850 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature