Novel strategy for activating gene expression through triplex DNA formation targeting epigenetically suppressed genes.

ABSTRACT: Triplex DNA formation is a useful genomic targeting tool that is expected to have a wide range of applications, including the antigene method; however, there are fundamental limitations in its forming sequence. We recently extended the triplex DNA-forming sequence to methylated DNA sequences containing ^5mCG base pairs by developing guanidino-dN, which is capable of recognizing a ^5mCG base pair with high affinity. We herein investigated the effect of triplex DNA formation using TFOs with guanidino-dN on methylated DNA sequences at the promoter of the RASSF1A gene, whose expression is epigenetically suppressed by DNA methylation in MCF-7 cells, on gene expression. Interestingly, triplex DNA formation increased the expression of the RASSF1A gene at the transcript and pro

SUBMITTER: Notomi R 

PROVIDER: S-EPMC11353075 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature