Dataset Information

Circulating miRNA Signaling for Fatty Acid Metabolism in Response to a Maximum Endurance Test in Elite Long-Distance Runners.

ABSTRACT: Maximal oxygen uptake (VO₂max) is a determining indicator for cardiorespiratory capacity in endurance athletes, and epigenetics is crucial in its levels and variability. This initial study examined a broad plasma miRNA profile of twenty-three trained elite endurance athletes with similar training volumes but different VO₂max in response to an acute maximal graded endurance test. Six were clustered as higher/lower levels based on their VO₂max (75.4 ± 0.9 and 60.1 ± 5.0 mL.kg^-1.min^-1). Plasma was obtained from athletes before and after the test and 15 ng of total RNA was extracted and detected using an SYBR-based 1113 miRNA RT-qPCR panel. A total of 51 miRNAs were differentially expressed among group comparisons. Relative amounts of miRNA showed a clustering behavior among groups regarding distinct performance/time points. Significantly expressed miRNAs were used to perform functional bioinformatic analysis (DIANA tools). Fatty acid metabolism pathways were strongly targeted for the significantly different miRNAs in all performance groups and time points (p < 0.001). Although this pathway does not solely determine endurance performance, their significant contribution is certainly achieved through the involvement of miRNAs. A highly genetically dependent gold standard variable for performance evaluation in a homogeneous group of elite athletes allowed genetic/epigenetic aspects related to fatty acid pathways to emerge.

SUBMITTER: Paulucio D

PROVIDER: S-EPMC11353293 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature

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Circulating miRNA Signaling for Fatty Acid Metabolism in Response to a Maximum Endurance Test in Elite Long-Distance Runners.

Paulucio Dailson D Ramirez-Sanchez Carlos C Velasque Rodolfo R Xavier Raphael R Monnerat Gustavo G Dill Adrieli A Silveira Juliano J Andrade Gabriella M GM Meirelles Flavio F Dornelas-Ribeiro Marcos M Kirchner Benedikt B Pfaffl Michael W MW Pompeu Fernando F Santos Caleb G M CGM

Genes 20240817 8

Maximal oxygen uptake (VO2max) is a determining indicator for cardiorespiratory capacity in endurance athletes, and epigenetics is crucial in its levels and variability. This initial study examined a broad plasma miRNA profile of twenty-three trained elite endurance athletes with similar training volumes but different VO2max in response to an acute maximal graded endurance test. Six were clustered as higher/lower levels based on their VO2max (75.4 ± 0.9 and 60.1 ...[more]

PMID: 39202447

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Project description:BackgroundDespite the positive effects of endurance training on the cardiovascular (CV) system, excessive exercise induces not only physiological adaptations but also adverse changes in CV system, including the heart. We aimed to evaluate the selected miRNAs expression based on bioinformatic analysis and their changes before and after an ultramarathon run.Materials and methodsCardiac tissue-specific targets were identified with the Tissue 2.0 database. Gene-gene interaction data were retrieved from the STRING app for Cytoscape. Twenty-three endurance athletes were recruited to the study. Athletes ran to completion (100 km) or exhaustion (52-91 km, median 74 km). All participants completed pre- and post-run testing. miRNAs expressions were measured both before and after the race.ResultsEnrichment analysis of the signaling pathways associated with the genes targeted by miRNAs selected for qRT-PCR validation (miR-1-3p, miR-126, miR-223, miR-125a-5p, miR-106a-5p, and miR-15a/b). All selected miRNAs showed overlap in regulation in pathways associated with cancer, IL-2 signaling, TGF-β signaling as well as BDNF signaling pathway. Analysis of metabolites revealed significant regulation of magnesium and guanosine triphosphate across analyzed miRNA targets. MiR-1-3p, miR-125a-5p, miR-126, and miR-223 expressions were measured in 23 experienced endurance athletes, before and after an ultramarathon wherein athletes ran to completion (100 km) or exhaustion (52-91 km, median 74 km). The expressions of miR-125a-5p, miR-126, and miR-223 were significantly increased after the race (p = 0.007, p = 0.001, p = 0.014, respectively). MiR-1-3p expression post-run showed a negative correlation with the post-run levels of high-sensitivity C-reactive protein (hs-CRP) (r = -0.632, p = 0.003). Higher miR-1-3p expression was found in runners, who finished the race under 10 h compared to runners who finished over 10 h (p = 0.001). Post-run miR-125a-5p expression showed a negative correlation with the peak lactate during the run (r = -0.576, p = 0.019).ConclusionExtreme physical activity, as exemplified by an ultramarathon, is associated with changes in circulating miRNAs' expression related to inflammation, fibrosis, and cardiac muscle function. In particular, the negative correlations between miR-125a-5p and lactate concentrations, and miR-1-3p and hs-CRP, support their role in specific exercise-induced adaptation. Further studies are essential to validate the long-term effect of these observations.

Project description:The pacing behaviors used by elite athletes differ among individual sports, necessitating the study of sport-specific pacing profiles. Additionally, pacing behaviors adopted by elite runners differ depending on race distance. An "all-out" strategy, characterized by initial rapid acceleration and reduction in speed in the later stages, is observed during 100 m and 200 m events; 400 m runners also display positive pacing patterns, which is characterized by a reduction in speed throughout the race. Similarly, 800 m runners typically adopt a positive pacing strategy during paced "meet" races. However, during championship races, depending on the tactical approaches used by dominant athletes, pacing can be either positive or negative (characterized by an increase in speed throughout). A U-shaped pacing strategy (characterized by a faster start and end than during the middle part of the race) is evident during world record performances at meet races in 1500 m, 5000 m, and 10,000 m events. Although a parabolic J-shaped pacing profile (in which the start is faster than the middle part of the race but is slower than the endspurt) can be observed during championship 1500 m races, a negative pacing strategy with microvariations of pace is adopted by 5000 m and 10,000 m runners in championship races. Major cross country and marathon championship races are characterized by a positive pacing strategy; whereas a U-shaped pacing strategy, which is the result of a fast endspurt, is adopted by 3000 m steeplechasers and half marathoners. In contrast, recent world record marathon performances have been characterized by even pacing, which emphasizes the differences between championship and meet races at distances longer than 800 m. Studies reviewed suggest further recommendations for athletes. Throughout the whole race, 800 m runners should avoid running wide on the bends. In turn, during major championship events, 1500 m, 5000 m, and 10,000 m runners should try to run close to the inside of the track as much as possible during the decisive stages of the race when the speed is high. Staying within the leading positions during the last lap is recommended to optimize finishing position during 1500 m and 5000 m major championship races. Athletes with more modest aims than winning a medal at major championships are advised to adopt a realistic pace during the initial stages of long-distance races and stay within a pack of runners. Coaches of elite athletes should take into account the observed difference in pacing profiles adopted in meet races vs. those used in championship races: fast times achieved during races with the help of one or more pacemakers are not necessarily replicated in winner-takes-all championship races, where pace varies substantially. Although existing studies examining pacing characteristics in elite runners through an observational approach provide highly ecologically valid performance data, they provide little information regarding the underpinning mechanisms that explain the behaviors shown. Therefore, further research is needed in order to make a meaningful impact on the discipline. Researchers should design and conduct interventions that enable athletes to carefully choose strategies that are not influenced by poor decisions made by other competitors, allowing these athletes to develop more optimal and successful behaviors.

Dataset Information

Circulating miRNA Signaling for Fatty Acid Metabolism in Response to a Maximum Endurance Test in Elite Long-Distance Runners.

Publications

Circulating miRNA Signaling for Fatty Acid Metabolism in Response to a Maximum Endurance Test in Elite Long-Distance Runners.

Similar Datasets

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