Assessing the Efficacy of Protease Inactivation for the Preservation of Bioactive Amphibian Skin Peptides.

ABSTRACT: The skin of amphibians is a rich source of peptides with a wide range of biological activities. They are stored in secretory granules in an inactive form. Upon stimulation, they are secreted together with proteases into the skin. Once activated, they rapidly exert their biological effects, including fighting microorganisms and predators, while their excess is immediately destroyed by the released proteases. To keep bioactive peptides in their initial form, it is necessary to inhibit these enzymes. Several inhibitors for this purpose have previously been mentioned; however, there has not been any reliable comparison of their efficiency so far. Here, we studied the efficiency of methanol and hydrochloric and formic acids, as well as phenylmethylsulfonyl fluoride, in the inhibition of nine fr

SUBMITTER: Samgina TY 

PROVIDER: S-EPMC11354720 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature